Paper ID: 52

DIVERSITY INDUCTION WITH GAMMA RAY IRRADIATION ON DENDROBIUM ODOARDI ORCHID

Authorship

T S Fathin1, S Hartati2,3, A Yunus 2,3*

1 Undergraduate Program in Agrotechnology Study Program, Faculty of Agriculture, Sebelas Maret University

2  Lecturer staff of the Agrotechnology Study Program, Faculty of Agriculture, Sebelas Maret University

3 Center of Biotechnology and Biodiversity, Research and Development, Sebelas Maret University

yunus@staff.uns.ac.id

Abstract

Orchid is an ornamental plant commodity that has potential to be developed. One species of orchid that is in demand is Dendrobium odoardi. The aims of this research is to determine the characteristics and morphological diversity of Dendrobium odoardi orchid irradiated gamma ray results and without gamma ray irradiation. This research was conducted in two places, the implementation of gamma ray irradiation at the PATIR-BATAN Laboratory (Pusat Aplikasi Teknologi Isotop dan Radiasi-Badan Tenaga Nuklir Nasional), Pasar Jumat, South Jakarta and subculture in Plant Physiology and Biotechnology Laboratory of Agriculture Faculty, University of Sebelas Maret Surakarta. The research was conducted start from March 2019 – January 2020. The research used a simple design of one factor, the dose of gamma ray irradiation with 5 levels, 0 Gy (control), 10 Gy, 15 Gy, 20 Gy, and 25 Gy. Each level of treatment was planted and observed 50 planlets. Observational data were analyzed descriptively by comparing observational data from Dendrobium odoardi orchids irradiated with control plants. The results showed that the induction of gamma ray irradiation at doses of 10 Gy, 15 Gy, 20 Gy, and 25 Gy resulted changes in morphological characters in Dendrobium odoardi orchid plant. The changes that occur are increasing plant height, increasing leaf width, decreasing number of roots, decreasing root length, change in leaf shape and change in leaf color. Changes in the morphological character of Dendrobium odoardi orchids produce plant diversity.

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