Paper ID: 53

GENETIC VARIABILITY OF PHAIUS AND DENDROBIUM ORCHIDS BASED ON MOLECULER MARKERS

Authorship

Wachidatul Linda Yuhanna1, Sri Hartati2,3*,  Sugiyarto4, Marsusi4

1)Department of Biology Education, Faculty of Teacher Training and Education, Universitas PGRI Madiun, Jalan Setia Budi No. 85 Madiun, Indonesia

2)Department of Agrotechnology, Faculty of Agriculture, Sebelas Maret University, Jl. Ir. Sutami 36 A, Surakarta 57126, Indonesia

3)Center of Biotechnology and Biodiversity Research and Development, Sebelas Maret University, Jl. Ir. Sutami 36 A, Surakarta 57126, Indonesia

4)Department of Biosciences, Postgraduate Program, Sebelas Maret University, Jl. Ir. Sutami 36 A, Surakarta 5712, Indonesia

tatik_oc@yahoo.com

Abstract

This study aimed to assess the variability and grouping degree of several species of orchids existing in Indonesia. The laboratory works were done in the Laboratory of Study Centre of Horticulture and Tropics (PHKT) of  IPB by analyzing the variability of orchids using RAPD. The plant materials were taken from the orchid collection at Kebun Raya Bogor. The relationship of 9 orchids were analyzed using the phenotype binary data and the DNA ribbon pattern which were assessed from amplification of 3 random primer RAPD. Cluster analysis was done under the program of NTSYSpc version 2.02 and UPGMA function SimQual. The study concluded that there were genetic variability and grouping among the species of Orchid,  the RAPD under primer of  OPA7, OPA 9, and OPA 19,  on the dendrogram based on Similarity coefficient of 0,40 showed that there were four group.  First group : Phaius tankervilliae (1), Phaius montanus (2) and Phaius callosus (3), group II : Dendrobium mirbelianum (5), Dendrobium lamellatum (6), Dendrobium liniale (8) and Dendrobium biggibum (9). Group III : Dendrobium anosmum (7). Group IV : Phaius ambionensis (4).

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