Paper ID: 45

CONSORTIUM BACTERIA DESIGN OF PATHOGENIC FUNGI DEVELOPMENT CONTROLLER FUSARIUM OXYSPORUM F. SP. CUBENSE (FOC)

Authorship

Y Effendi*, A El Hadi and A Pambudi

Department of Biology, Universitas Al Azhar Indonesia.

Jl. Sisingamangaraja No. 2, Jakarta Selatan 12110, Jakarta, Indonesia

effendiy@uai.ac.id

Video Presentation

Abstract

Banana plants (Musa sp.) on PT Perkebunan Nusantara (PTPN) VIII, Parakansalak, Sukabumi West Java plantation were indicated infected by panama wilt was caused by Fusarium oxysporum f.sp. cubense (Foc). In this case, rhizosphere bacteria is one of a kind of organism, which can be used as a biocontrol to induct the systemic resistance on the plants. This research was done to obtain the designation and evaluate the potential of bacteria consortium, which has the ability to slowed down the growth of the Foc. The candidates of non -pathogenic Foc inhibitor bacteria are being selected through the antagonistic test, pathogenic test, and compatibility test. The effectiveness of inhibition selected bacteria will be tested in-vitro through the greenhouse-scale of suppressive soil experiments. The result shows that the Bacillus cereus strain CCM 2010 and BS 3-4B has the highest percentage of inhibition to the Foc (25.68 – 29.02%). Non-pathogenic bacteria with a percentage of inhibition above 20%, consists of 3 consortia by compatibility testing. 1st consortium (BS 3-4B, Bacillus cereus strain CCM 2010, Staphylococcus arlettae strain ATCC 43957, Bacillus cytotoxicus strain NVH 391-98 and Bacillus pseudomycoides strain NBRC 101232), 2nd consortium (Bacillus cereus strain CCM 2010 and Lysinibacillus xylanilyticus strain XDB9), and 3rd consortium (Lysinibacillus xylanilyticus strain XDB9 dan Bacillus pseudomycoides strain NBRC 101232). Results of the observation show that the infected control plant by Foc having the yellow symptom on the day-3. Meanwhile, the infected plants with the addition of singular bacteria and consortium bacteria showing the symptoms on the day-5 and day-7.

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