Paper ID: 56

GENETIC CHARACTERIZATION OF ILER (PLECTRANTHUS SCUTELLARIOIDES (L.) R. Br.) BASED ON RAPD MOLECULAR MARKER

Authorship

Dyah Subositi1, Dina Rosdiana2, Alice Yuniaty2, Dian Susanti1, Anshary Maruzy1, Nur Rahmawati W1

1 Medicinal Plant and Traditional Medicine Research and Development Center, NIHRD-Ministry of Health, Tawangmangu, Central Java

2 Faculty of Biology, Jenderal Soedirman University, Purwokerto, Central Java

dyah.subositi@gmail.com

Video Presentation

Abstract

Iler (Plectranthus scutellarioides (L.) R. Br.) is an ornamental plant and also used as medicinal plant. The plant is one of medicinal plant for anti hemorrhoids Jamu Saintifik herbal formula in B2P2TOOT Indonesian Ministry of Health. This will lead to commercialization opportunities in herbal drug industry that recently has become increasingly popular. With the emergence of substitution and adulteration of herbal drug and the lack of information on coleus genetic diversity which may result in misidentification, this research was conducted to determine Iler genetic characteristics using RAPD markers. The genomic DNA was extracted from 15 accessions of Iler and 3 accessions from genus Plectranthus as an outgroup. Six selected RAPD primers were used in amplification. Dice similarity index was used to calculate similarity index followed by cluster analysis and dendrogram contruction using Unweighted Pair Group Method with Arithmetic Mean (UPGMA). The results showed 52 DNA fragments were produce within the size range 293-2.667 bp and polymorphism level among accessions at 95,31%. Cluster results results in genetic diversity ranging from 40,45-97,87% and a dendogram that shows clustering trend of Iler accessions based on the similarity of morphological characters such as leaf shape and color. Specific bands of Iler for future authentication purpose are also determined with the band sizes as follows: OPA-1 (800 and 1246 bp), OPA-18 (388 bp), OPB-1 (315 and 1159 bp), OPC-2 (364 bp), OPD-2 (1166 bp), OPH-13 (1002 and 1493 bp).

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