Paper ID: 78

THE EFFECT OF BENZILAMINOPURINE AND KINETIN FOR SHOOT MULTIPLICATION OF INDIGOFERA (INDIGOFERA ZOLLINGERIANA MIQ.) BY IN VITRO CULTURE

Authorship

Juwartina Ida Royani1, Widia Sri Fatma2, Susiyanti2, Andi Apriany Fatmawaty2

1Badan Pengkajian dan Penerapan Teknologi, Gd. Laptiab 610 Kawasan PUSPIPTEK Serpong Tangerang 15314 Indonesia

2Jurusan Agroekoteknologi Fakultas Pertanian-Universitas Sultan Ageng Tirtayasa, Jalan Raya Jakarta Km 4, Panancangan, Cipocok Jaya, Kota Serang, Banten 42124

juwartina.ida@bppt.go.id

Video Presentation

Abstract

Indigofera (Indigofera zollingeriana Miq.) is one of the legume trees that are useful as forage and have advantages in the production and quality of forage compared to other types of legumes. This forage contains high protein and mineral sources, good fiber structure and high digestibility value. Nutrient contents of Indigofera are crude protein (31%) with 76% of dry matter digestibility (DMD) and 83% of organic matter digestibility (ODM). Indigofera has low germination due to thick seed coat and fungal invasion during germination. Propagation by in vitro culture promise to multiply superior seeds from Indigofera. The aim for this research was to determine the effect of Benzylaminopurine and Kinetin for multiply of shoots of Indigofera. The research was designed using Completely Randomized Design (CRD) with 2 factor (BAP and Kinetin concentration) and repeated 3 times. BAP concentration consists of four levels, ie:  0, 1, 1.5 and 2 mg/L and Kinetin concentration consists of four levels too, ie: 0, 1, 2 and 3  mg/L. The results showed that BAP gave the best results for number of shoots parameter at any concentration. But compact callus appears in basal of shoots at BAP (1.5 mg/L and 2 mg/L) media. Whereas media with Kinetin showed no significant effect on all parameters and there was no interaction between BAP and Kinetin.

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