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Paper ID: 107

Analysis of bacterial community from the rhizosphere of shallots (Allium ascalonicum L.), in Brebes, Central Java using Next Generation Sequencing (NGS) approach

R Rosariastuti1*, Sutami1, Sumani1, and S Hartati1

1Faculty of Agriculture, Universitas Sebelas Maret, Indonesia

Email: retnobs@staff.uns.ac.id

Brebes is the largest shallot center in Indonesia. This area contributes 18.5 % of national production or 57 % of production in Central Java. In their care, farmers spray about 63-93% of pesticides regularly every 3-7 days. This requires improvement in technology and the use of bacteria in it. This research was conducted to investigate the diversity of soil bacteria in two types of land use as initial information to optimize the role in overcoming pesticide contamination and increasing the productivity of shallots in the future. The bacterial community in the shallot field (KB2) and secondary forest (KB4) in this study was observed using the Next Generation Sequencing technique. Identification using FLASH (V1.2.7) and QIIME (V1.7.0) analysis. The results showed that the soil bacterial communities in KB2 were 7 phyla while KB4 were 8 phyla. In order of largest to smallest relative abundance in KB2 including Proteobacteria (0.970%), Actinobacteria (0.015%), Firmicutes (0.014%), Fusobacteriota (0.001%), Desulfobacterota (0.0003%), Bacteroidota (0.0001%), and others (0.011%). In KB4 there are 8 phyla namely Elusimicrobiota (6.385%), Cyanobacteria (3.192%), Proteobacteria (0.9861%), Actinobacteriota (0.0041%), Firmicutes (0.0089%), Desulfobacterota (0.0001%), Bacteroidota (0.0002%), and Others (0.0003%).

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