¹ Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta, Indonesia

² Center of Biotechnology and Biodiversity, Research and Development, Universitas Sebelas Maret, Indonesia

Email: baambang_p56@staff.uns.ac.id

Sambiloto contains andrographolid which can increase the body’s immune system. The demand for sambiloto in Indonesia has increased, but is not matched by production efforts. The fulfillment of the need for sambiloto still depends on side cultivation and harvesting from nature, so that it will reduce the availability of germplasm and germplasm. This study aims to obtain the composition of planting media, watering time intervals, and the right interaction as a technology innovation for bitter cultivation. The experiment used a factorial completely randomized design (CRD) with two treatments. The first factor is the composition of the growing media soil, soil and cow manure, soil and vermicompost, and soil and goat manure. The second factor is the treatment of watering intervals every 3 days and every 4 days. The results showed that there was no interaction between the composition of the growing media and the watering time interval. Soil media and vermicompost gave the best results on plant height, number of leaves, number of primary branches, fresh and dry weight of plants, and andrographolid content. Watering every 3 and 4 days gave the same effect on the growth of bitter. The highest andrographolid (8.03%) was produced by soil media and vermicompost fertilizer.