Paper ID: 59
GC-MS analysis of bioactive compounds from leaves extract of Melastoma malabatrichum, Clidemia hirta, Chromolaena odorata, and Ageratum conyzoides
Nurul Hana Ismail1, Nur’ Amira Hamid1*, Wan Zuraida Wan Mohd Zain1,Fazlena Hamzah2 and Siti Noor Hajjar Md Latip1,3
1 Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA, Jasin Campus, Melaka, Malaysia
2 Faculty of Chemical Engineering, Universiti Teknologi MARA, Shah Alam Campus, Selangor, Malaysia
3 Sustainable Crop Protection Research Group, Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA, Shah Alam Campus, Selangor, Malaysia
Melastoma malabathricum, Clidemia hirta, Chromolaena odorata, and Ageratum conyzoides are classified under broad-leaved and categorized as potential weeds as they have high nutritive value, also rich in chemical compounds for various bioactivities. The present investigation was carried out to determine the possible bioactive components of these weeds using GC-MS analysis. Extraction from the leaves of these weeds was done using different solvents like ethanol, acetone, and ethyl acetate. The GC- MS analysis result showed the presence of bioactive compounds in each extract of the four weeds. The results showed that M. malabatchricum leaf extract contained Pentadecanoic acid, 14-methyl-, methyl ester and 9-Octadecenoic acid, methyl ester, (E)- for ethanol extract, 1,4-Benzodiazepin-2(1H,3H)-one, 7-chloro-1-ethyl-5-phenyl- and Spiro[bicyclo[2.2.1]hept-5-ene-2,1′-cyclopropane], 1,4,5,6,7,7-hexachloro- for acetone extract and 1-Triethylsilyloxydodecane and 2,3-Dihydroxypropyl elaidate for ethyl acetate extract. C. hirta showed presence of 3,7,11,15-Tetramethyl-2-hexadecen-1-ol and 3-Hexadecyne for ethanol extract, 2-Butyl-1,2-azaborolidine and 1,2-15,16-Diepoxyhexadecane for acetone extract and 3,7,11,15-Tetramethyl-2-hexadecen-1-ol and Estra-1,3,5(10)-trien-17á-ol for ethyl acetate extract. C. odorata showed the presence of 2-(7-Hydroxymethyl-3,11-dimethyl-dodeca-2,6,10-trienyl)-[1,4]benzoquinone and 5,19-Cyclo-5á-androst-6-ene-3,17-dione for ethanol extract, Pentadecanoic acid, 14-methyl-, methyl ester and 11-Octadecenoic acid, methyl ester for acetone extract and Methyl 9,12-epithiostearate and 9-Octadecenoic acid (Z)-, 2,3-dihydroxypropyl ester for ethyl acetate extract. A. conyzoides showed presence of Bicyclo[3.3.1]non-2-en-9-ol, anti- and à-Caryophyllene for ethanol extract, Tricyclo[184.108.40.206(2,6)]heptane, 1,7-dimethyl-7-(4-methyl-3-pentenyl)-, (-)- and à-Caryophyllene for acetone extract and Tricyclo[220.127.116.11(2,6)]heptane, 1,7-dimethyl-7-(4-methyl-3-pentenyl)-, (-)- and 6-Methyl-6-hepten-4-yn-2-ol for ethyl acetate extract. Some types of compounds found are known to have the potential as antibacterial, antiviral, antioxidant, insecticidal, and anti-inflammatory.