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Paper ID: 81

Genetic diversity of strawberry (Fragaria x ananassa) var. Earlibrite mutant as revealed by ISSR molecular marker induced by gamma rays irradiation

H Arisah1,2, D Saptadi2, S Ashari2, D Agisimanto3, and F Yulianti3*

1Indonesian Citrus and Subtropical Fruits Research Institute, Jl. Raya Tlekung No.1, Kota Batu, Indonesia 65327

2Faculty of Agriculture, Universitas Brawijaya, Jl. Veteran, Malang, Indonesia 65145

3Horticulture and Plantation Research Center, National Research and Innovation Agency, Jl. Raya Surabaya – Malang Km. 65, Sembung Lor, Parerejo, Kec. Purwodadi, Pasuruan, East Java 67163

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Strawberry plantlets (Fragaria X ananassa) cv Earlibrite were irradiated by gamma rays to induce genetic variability for resistance to abiotic stress improvement. The aim of this research was to identify DNA polymorphism among the ‘Earlibrite’ strawberry mutants through ISSR marker. For those purposes, the population of M1V0 strawberry plantlets was irradiated by gamma rays at 20 Gy (optimum dosages of LD50) and then sub-cultured on MS medium enriched with 2 µM 6-BAP and 0,13 µM 1-NAA.  The genetic variation of the mutant was molecularly analyzed using ISSR marker.  The analysis was carried out on M1V0 mutant plantlets.  The results showed, ISSR markers produced 192 scorable bands, of which 93,36% were polymorphic. The PIC values for the 15 primers ranged from 0.40 to 0.50 with an average of 0.48 per primer. The marker index (MI) of the primers ranged from 2.38 to 4.85.  According to the Jaccard similarity index, the genetic variation of mutant individuals was 2.74% to 87.04%. Cluster analysis using ISSR markers grouped the Earlibrite strawberry mutant genotypes into five distinct groups.  The current study demonstrated that gamma irradiation generates a sufficient number of induced mutations and that ISSR analysis offered a useful molecular marker analysis for identifying mutants.

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