Skip to content

Paper ID: 82

Screening of simple sequence repeats (SSR) primers from mutated Indigofera zolligeriana plants

J I Royani1,2, O B Br Sinaga3, K N Aliyah4, D Hardianto1, T Agustina1, M N Rofiq1, Kristamtini1, T Handayani1, W Puspitasari1, Sudarsono2, L Abdullah5, and S I Asiyah2

1National Research and Innovation Agency (BRIN) – Indonesia

2Plant Genetics and Plant Breeding, Agronomy and Horticulture, Study Program-Faculty of Agriculture, IPB University-Indonesia.

3Faculty of Mathemathic and Science Universitas Riau – Indonesia

4Faculty of Biology Universitas Djendral Soedirman – Indonesia
5Animal Production and Technology, Faculty of Animal Husbandry, IPB University-Indonesia

Email: juwartina.ida.royani@brin.go.id

The mutated plants from Indigofera zolligeriana were screened using 15 SSR primers, to know the polymorphic DNA profile of mutated plants. Primers used for screening were R1, R2, R3, R4, R5, R6, R7, R8, R9, R10, R11, R12, R13, R14, and R15. For screening these primers we used 12 DNA genomes of mutated I. zolligeriana plants. DNA genomes from 12 mutated plants were isolated using Wizard® Genomic DNA Purification Kit. Amplification of 12 DNA genomes using multiplex PCR method followed by optimization of the method to obtain the best bands profile. The result showed that from 15 primers, 10 primers were polymorphic bands and 5 primers were monomorphic bands. Primers that showed polymorphic bands will be used to amplify another mutated DNA genome.

Leave a Reply

Your email address will not be published.